Hybridizes, washes and scans Affymetrix arrays that quantify relative changes in RNA/miRNA and DNA

Automatically performs hybridization and wash steps required for NanoString cartridges, prior to scannning

Specialized accessories (holders, washer) and scanner required to perform methylation array analysis

Calibrated scanner that measures spectral excitation from dyes used in commercial ELISA kits

Measures electroluminescent emission from probes utilized in high-sensitivity, multiplexed protein assays

Quantify the distribution of all lengths of nucleic acids present in a sample; Bioanalyzer required for miRNAs

Reproducibly cuts DNA into specific sizes required for downstream procedures such as ChipSeq, seq etc.

Measures and automatically separates different sizes of long/HMW DNA/RNA for downstream long-read seq

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Includes sequencer setup/run, qPCR, dPCR (all formats). Core staff develop or troubleshoot methods to support investigator objectives. For one-time projects or to establish protocols for adoption by the PI's lab team. Routine lab work such as sample organization, biorepository creation, IPA and other simple analyses etc. Advanced bioinformatics and/or statistical analyses are available through those respective CNRI cores. 

Tape Station and/or Bioanalyzer analysis.

Quality assesment of integrity or "intactness" of nucleic acids.

Strongly advised prior to sequencing applications       

Isolation from "purple=top" or PAX tubes. Isolation from tissue, saliva and other materials is available as a custom service    

Determines the purity of an isolated sample with NanoDrop/Spec.

Contaminating proteins and chemicals can interfere with downstream processes    

Qubit and specific intercalating dyes are used to quantify nucleic acids.

Accuracy is superior to NanoDrop/Spec and required for sequencing applications.    

Illumina Methylation EPIC v 2.0 genome-wide (>935K CpG sites) methylation screening tool.

Quantitatively interrogates at 1-bp resolution, independent of read depth.

Accepts multiple DNA sample types, including FFPE, with greater scalability and lower cost vs sequencing.

Dedicated bench and office space for temporary use (days to several months). Small benchtop and other general equipment, chemical and biohazard use included . Core staff provide guidance, orientation and suggestions as needed. Training should be scheduled as a separate service.

Detect expression of low abundance transcripts and/or splice variants at lower cost than comparable sequencing. No rRNA or globin mRNA reduction required; FFPE and limited (100 pg) samples can be examined. Relatively fast and easy analysis  by biologists with free software .

nCounter inventoried or custom panels for gene expression analysis by direct digital counting. Gene Fusion, CNV or TagSet DNA panels.

Variety of library preps (stranded, 3' etc) using well-established Illumina and Lexogen protocols.

Sequencing cost is separate and matched to project needs.     

Gene Expression, ATAC or Multiome. Investigator provides pre-cleaned, counted preps to the core. Core creates gems, libraries and prepares for sequencing. Sequencing cost is separate and matched to project needs; Order seperately as a custom service.

Luminex ELISA or MSD ECL ELISA for increased sensitivity and dynamic range. Single, multiplexed (up to 54 plex) and/or custom designed assays. V-plex validation through MSD for consistent measurements over time/multiple assays.

PCR based SNP analysis on isolated DNA; design of probes is available. We do not perform Sanger seq-but can provide guidance and resources for this.

One-on-one or small groups.

Any method we support and/or general best lab practises.

Requestor supplies project-specific reagents.